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Restriction Enzymes
This tool screens for the following restriction enzyme sites:
Configuring Restriction Enzymes
Define custom restriction sites if needed.
Restriction sites must be 5 - 15 valid characters.
Toggle or add restriction sites to be avoided.
Selected:
Enter items as shown below. Name and sequence are required.
To optimze a portion of the sequence, enter start and end locations after the sequence. Start and end locations are inclusive.
To optimize the whole sequence, leave the start and end locations blank.
Reading frame selection not available for FASTA entry. Amino acid sequences are entered using single-letter abbreviations.
To optimze a portion of the sequence, enter start and end locations after the sequence. Start and end locations are inclusive.
To optimize the whole sequence, leave the start and end locations blank.
Reading frame selection not available for FASTA entry. Amino acid sequences are entered using single-letter abbreviations.
Results
Sequence passes initial screening.
Total Complexity Score:
Complexity Description
Score