gene_expression

PrimerQuest® Tool

Effortlessly design PCR and qPCR assays for any species

  • ✔ Probe-based qPCR assays
  • ✔ Intercalating dye (SYBR® Green) qPCR assays
  • ✔ PCR and qPCR primers spanning exon junctions
  • ✔ Sequencing primers
  • ✔ Multiplexed PCR and qPCR

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Related Article:

Design efficient PCR and qPCR primers and probes using online tools

The basics of designing customized primers and probes at IDT. Be sure to read the section, “The PrimerQuest Tool in 4 steps”.

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Design PCR and qPCR Primers with PrimerQuest

This short tutorial demonstrates how to quickly design PCR and qPCR primers for multiple sequences using PrimerQuest.

Key features of the PrimerQuest® Tool

Input target sequences manually (FASTA), or download them using GenBank ID or Accession Number
Enter sequences individually or in bulk (up to 50 sequences) via Excel
Customize multiple design parameters, including primer and probe characteristics, exon junctions, amplicon length, and more
Input target sequences manually (FASTA), or download them using GenBank ID or Accession Number
Enter sequences individually or in bulk (up to 50 sequences) via Excel
Customize multiple design parameters, including primer and probe characteristics, exon junctions, amplicon length, and more

Design considerations for qPCR assays

Quantitative PCR (a.k.a. qPCR or real-time PCR) is one of the most commonly used techniques in molecular life sciences. It is used for multiple applications including gene expression, SNP genotyping, and copy number variation. However, knowing how to achieve the best results is not always straight-forward. In this webinar on 5′ nuclease (primer- and probe-based) qPCR assay design and experimental setup, Senior Scientific Applications Specialist, Dr Erik Wendlandt, provides guidelines for using design parameters such as Tm, GC content, amplicon size, and primer and probe location. Dr Wendlandt describes several free, online tools, including BLAST, that can aid in primer/probe design and analysis. General considerations for droplet digital PCR (ddPCR) are included.

Related Article:

PrimerQuest Tool: From basic to highly customizable designs

In-depth tips on how to get the most out of the PrimerQuest Tool, with discussion of a variety of common qPCR applications.

Contact us

Call, email, or web chat us today.

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PrimerQuest is my go-to tool. I use it every day in my research, and it saves me time because I order the right material so I don't have to repeat experiments due to mistakes made in nearest neighbor thermodynamic parameters, where R is the ideal gas constant (1.987 calK-1mole-1).

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- Published Principal Investigator

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