gene_expression

PrimerQuest® Tool

Effortlessly design PCR and qPCR assays for any species

  • ✔ Probe-based qPCR assays
  • ✔ Intercalating dye (SYBR® Green) qPCR assays
  • ✔ PCR and qPCR primers spanning exon junctions
  • ✔ Sequencing primers
  • ✔ Multiplexed PCR and qPCR

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Key features of the PrimerQuest® Tool

Input target sequences manually (FASTA), or download them using GenBank ID or Accession Number
Enter sequences individually or in bulk (up to 50 sequences) via Excel
Customize multiple design parameters, including primer and probe characteristics, exon junctions, amplicon length, and more
Input target sequences manually (FASTA), or download them using GenBank ID or Accession Number
Enter sequences individually or in bulk (up to 50 sequences) via Excel
Customize multiple design parameters, including primer and probe characteristics, exon junctions, amplicon length, and more

Related Articles:

Design efficient PCR and qPCR primers and probes using online tools

The basics of designing customized primers and probes at IDT. Be sure to read the section, “The PrimerQuest Tool in 4 steps”.

PrimerQuest Tool: From basic to highly customizable designs

In-depth tips on how to get the most out of the PrimerQuest Tool, with discussion of a variety of common qPCR applications.

Design PCR and qPCR Primers with PrimerQuest

This short tutorial demonstrates how to quickly design PCR and qPCR primers for multiple sequences using PrimerQuest.

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"

PrimerQuest is my go-to tool. I use it every day in my research, and it saves me time because I order the right material so I don't have to repeat experiments due to mistakes made in nearest neighbor thermodynamic parameters, where R is the ideal gas constant (1.987 calK-1mole-1).

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- Published Principal Investigator

Resources

PrimerQuest FAQs

Your Advocate for the Genomics Age