Please note that we recently updated our default parameter set to “qPCR” to align with the most commonly used OligoAnalyzer Tool setting.
If you would like to switch the parameters to the previous default of “SpecSheet”, this can be selected from the “Parameter sets” dropdown menu.

Example Files

Input - Either paste in your sequences directly into the text area or upload a file (xlsx or fasta)

Parameter sets

Target type

Oligo Conc

µM

Na⁺ Conc

Mg⁺⁺ Conc

dNTPs Conc

NAME	SEQUENCE	COMPLEMENT	LENGTH	GC CONTENT	MELT TEMP	MOLECULAR WEIGHT	EXTINCTION COEFFICIENT	nmole/OD260	µg/OD260	ΔG (kcal.mole^-1)	T_m (^oC)
Processing

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Batch Analysis Analyze up to 200 sequences at a time.

Example Files

Batch Analysis
Analyze up to 200 sequences at a time.