gene_expression

{Tool Name}

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Design PCR and qPCR Primers with PrimerQuest

This short tutorial demonstrates how to quickly design PCR and qPCR primers for multiple sequences using PrimerQuest.

Key features/benefits of the {Tool Name} tool.

Input target sequences manually (FASTA), or download them using GenBank ID or Accession Number
Enter sequences individually or in bulk (up to 50 sequences) via Excel
Customize multiple design parameters, including primer and probe characteristics, exon junctions, amplicon length, and more
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PrimerQuest is my go-to tool. I use it every day in my research, and it saves me time because I order the right material so I don't have to repeat experiments due to mistakes made in nearest neighbor thermodynamic parameters, where R is the ideal gas constant (1.987 calK-1mole-1). "

- Published Principal Investigator

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