MGB SNP Genotyping Assays

MGB (Minor Groove Binder) probes have significantly improved the number and composition of SNP targets that can be analyzed by enabling the genotyping of AT-rich sequences [Belousov YS, Welch RA et al. 2004, Hum Genomics, 209–217]. The incorporation of MGBs stabilizes probe-target hybridization and increases melting temperature (Tm), allowing the use of shorter probes, which are better suited for allelic discrimination.

IDT MGB SNP Genotyping Assays offer a simple, low-cost, high-performance genotyping solution. These assays include 2 primers and 2 PrimeTime MGB Probes that incorporate a 5′ reporter and a 3′ non-fluorescent quencher (NFQ), which reduces background signal and improve quantification. Multiple assay sizes are available. The Mini Assay (100 rxn) size is ideal for digital PCR (dPCR) platforms.


MGB SNP Genotyping Assays

Each assay kit includes 2 primers and 2 probes (5' SUN/ 3' MGB-NFQ and 5' 6-FAM/3' MGB-NFQ). Reaction size based upon use in a 20 µL final volume. Typically ships within 5-7 business days.


Frequently asked questions