The xGen Duplex Seq Adapters—Tech Access contains an equimolar pool of duplexed adapters, with each strand containing a 3 bp unique molecular identifier (UMI; Figure 1). The adapters are compatible with T-A ligation–based library preparation
kits. The adapter pool is normalized to 2 nmol, delivered in a tube, and provided at a concentration of 15 µM in 30 mM HEPES, pH 7.5; 100 mM potassium acetate. Nuclease-Free Duplex Buffer (catalog # 11-01-03-01) is recommended for dilution, if necessary.
xGen Duplex Seq Adapters—Tech Access does not include the full Illumina P5 and P7 adapter sequences or sample indexes necessary to generate sequencing-ready libraries. P5 and P7 sample indexing primers must be ordered separately using the sample indexing primer order form. These primers, each bearing a unique index, must be used in PCR amplification with xGen Duplex Seq Adapters to establish full-length adapters that include dual UMIs and sample indexes.
Use xGen Duplex Seq Adapters—Tech Access with xGen Universal Blockers–TS Mix to improve on-target capture during targeted sequencing.