Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is the PrimeTime Gene Expression Master Mix compatible with different real-time PCR instruments or systems?

Yes. The PrimeTime™ Gene Expression Master Mix has been successfully used in qPCR experiments run on a QuantStudio™ 7 Flex, 7900HT, and 7500 (all Thermo Fisher Scientific) instrument, as well as a CFX384™ (BioRad), and a LightCycler® 480 (Roche) instrument. These experiments used the cycling conditions provided in the PrimeTime™ Gene Expression Master Mix protocol.

Note: Reference dye is provided in a separate tube, so you can add it to the master mix at the levels required by your real-time PCR instrument.


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