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Tips for working with gBlocks Gene Fragments

While the ever-increasing availability of genomic sequence information simplifies the process of obtaining target DNA fragments, especially using PCR, there are still multiple, labor-intensive steps to generating your DNA constructs. You can eliminate many of these steps by substituting custom-synthesized, double-stranded DNA fragments in place of DIY constructs. gBlocks™ Gene Fragments are sequence-verified fragments that can be used in any application requiring double-stranded DNA. Ann Tigges, Synthetic Biology Business Coordinator at IDT, describes what to do when you receive your gBlocks Gene Fragments. Included is practical information on how to resuspend, quantify, and calculate copy number.

Published on: May 07, 2018